Anti-Double Stranded DNA Antibody (ds-DNA) ELISA Kit

Short Description:

Anti-dsDNA antibodies, also known as anti-natural DNA antibodies, are a specific type of antinuclear antibody (ANA) with critical clinical relevance. They are predominantly associated with systemic lupus erythematosus (SLE), where nearly all patients exhibit elevated levels of these antibodies. Accumulating evidence suggests that anti-dsDNA antibodies play a significant role in the pathogenesis of SLE, particularly in driving tissue damage through immune complex-mediated mechanisms.

In affected individuals, large DNA molecules can circulate in the bloodstream or adhere to the microvasculature of various organs—including the kidneys, lungs, and brain. These DNA molecules then bind to anti-dsDNA antibodies in the blood, forming circulating immune complexes that deposit in tissues, triggering inflammatory responses and subsequent organ injury. This mechanism is especially prominent in lupus nephritis, where over 50% of patients show higher circulating DNA concentrations compared to healthy individuals. However, the precise reasons and molecular pathways underlying this elevation remain poorly understood, highlighting a key area for future research.

Clinically, the titer of anti-dsDNA antibodies is widely recognized to correlate with disease activity: titers rise during active disease phases and decrease as the condition improves. Normal reference ranges vary across regions due to differences in detection methods, but a binding rate exceeding 20% is generally considered clinically significant for supporting diagnosis and monitoring disease progression. As research advances, it is anticipated that these unresolved questions will be gradually addressed, enhancing our understanding and management of SLE.

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Product Detail

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Principle

This kit is designed for the qualitative and semi-quantitative detection of anti-double-stranded DNA (anti-dsDNA) antibodies in human serum or plasma samples, utilizing an enzyme-linked immunosorbent assay (ELISA) format. The core component of the kit is polystyrene microwell strips pre-coated with highly purified double-stranded DNA (dsDNA) antigen, which serves as the specific binding target for the antibodies of interest.

The detection process begins with adding the serum or plasma specimens to the pre-coated wells. During this step, specific anti-dsDNA antibodies present in the samples—primarily IgG subclass antibodies (dsDNA-IgG-Ab) and a small portion of IgM subclass antibodies (dsDNA-IgM-Ab)—bind specifically to the immobilized dsDNA antigen on the solid phase. Unbound components, such as non-specific proteins and other antibodies, are then removed by thorough washing to minimize background interference.

In the second step, horseradish peroxidase (HRP)-conjugated anti-human IgG secondary antibody is added, which specifically recognizes and binds to the IgG-type anti-dsDNA antibodies already attached to the solid-phase antigen, forming a stable (dsDNA Ag)-(dsDNA-IgG)-(anti-human IgG-HRP) immune complex. A subsequent washing step removes any unbound HRP conjugate, ensuring only specifically bound enzyme remains.

Next, a chromogenic solution containing TMB substrate is added. The HRP in the immune complex catalyzes the oxidation of TMB, generating a blue product. Adding 50μl of stop solution halts the reaction, converting the blue color to a stable yellow. Finally, the absorbance of each well is measured using a microplate reader, with the absorbance value directly reflecting the presence and relative concentration of anti-dsDNA IgG antibodies in the sample.

Product Features

High sensitivity, specificity and stability

Product Specification

Principle	Enzyme linked immunosorbent assay
Type	Indirect Method
Certificate	NMPA
Specimen	Human serum / plasma
Specification	48T / 96T
Storage temperature	2-8℃
Shelf life	12 months