Anti-Islet Cell (ICA) Antibody ELISA Kit

Short Description:

This kit is designed for the qualitative in vitro detection of anti-islet cell antibody (ICA) levels in human serum. Clinically, it is mainly used as an auxiliary diagnostic tool for Type 1 Diabetes Mellitus (T1DM).

Islet cell antibodies are autoantibodies that target antigens on the surface or inside pancreatic islet cells, particularly β cells. Their presence is closely associated with autoimmune damage to islet cells, which is a key pathological feature of T1DM. In the early stages of T1DM, even before obvious clinical symptoms such as hyperglycemia appear, ICA can often be detected in the serum, making it an important early immune marker for the disease.

For individuals with a family history of diabetes or those showing pre-diabetic symptoms, detecting ICA levels helps assess the risk of developing T1DM. Additionally, in patients with unclear causes of hyperglycemia, ICA testing aids in differentiating T1DM from other types of diabetes, thereby guiding the formulation of appropriate treatment plans. By monitoring changes in ICA levels, it can also provide reference for evaluating the progression of islet cell damage and the effectiveness of intervention measures.

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Product Detail

Product Tags

Principle

This kit detects islet cell antibodies (ICA) in human serum samples based on the indirect method, with purified islet cell antigens used as the coating antigen.

The testing procedure begins by adding the serum sample to reaction wells pre-coated with the antigen, followed by incubation. If ICA is present in the sample, it will specifically bind to the coated islet cell antigens in the wells, forming stable antigen-antibody complexes. Unbound components are then removed through washing to ensure the accuracy of subsequent reactions.

Next, enzyme conjugates are added to the wells. After a second incubation step, these enzyme conjugates bind to the existing antigen-antibody complexes. When TMB substrate solution is introduced, the enzyme in the complex catalyzes a reaction with TMB, resulting in a visible color change. Finally, a microplate reader is used to measure the absorbance (A value), which allows for the determination of ICA levels in the sample based on the intensity of the color reaction.

Product Features

High sensitivity, specificity and stability

Product Specification

Principle	Enzyme linked immunosorbent assay
Type	Indirect Method
Certificate	NMPA
Specimen	Human serum / plasma
Specification	48T / 96T
Storage temperature	2-8℃
Shelf life	12 months