Anti-Ovarian (AO) Antibody ELISA Kit

This kit detects anti-ovarian antibodies (IgG) in human serum samples based on the indirect method, with purified ovarian membrane antigens used for pre-coating the microwells.

The testing process starts with adding the serum sample to the antigen-precoated reaction wells for incubation. If anti-ovarian antibodies are present in the sample, they will specifically bind to the pre-coated ovarian membrane antigens in the microwells, forming stable antigen-antibody complexes. Unbound components are then removed to ensure detection accuracy.

Next, horseradish peroxidase (HRP)-labeled mouse anti-human IgG antibodies are added to the wells. After a second incubation, these enzyme-labeled antibodies bind specifically to the anti-ovarian antibodies in the existing antigen-antibody complexes, forming a complete "antigen-antibody-enzyme label" immune complex.

Finally, TMB substrate solution is added. The HRP in the complex catalyzes a chemical reaction with TMB, producing a visible color change. The absorbance (A value) of the reaction solution is measured using a microplate reader, and the presence or absence of anti-ovarian antibodies in the sample is determined based on the absorbance result.