Anti-sperm (AS) Antibody ELISA Kit

Short Description:

Sperm, as a unique antigen, can trigger an immune response and produce anti-sperm antibodies (ASAb) when in contact with the immune system. Sperm antigens are hidden antigens. Normally, in males, the blood-testis barrier and immunosuppressive factors in semen prevent sperm antigens from contacting the immune system. In females, the enzyme system in reproductive organs degrades sperm antigens.

When males suffer from prostatitis, mumps, etc., or have testicular damage or poisoning, the blood-testis barrier is destroyed. Reduced immunosuppression in semen allows sperm antigens to enter the bloodstream, inducing autoantibodies and male infertility. In females, enzyme system defects, reduced semen immunosuppression, infections, or menstrual intercourse may preserve sperm antigens, inducing ASAb and interfering with sperm movement.

ASAb impairs sperm movement, preventing them from passing through cervical mucus, and reduces their ability to penetrate eggs. It causes 10%-30% of unexplained female infertility and may lead to poor embryo development or miscarriage.

Send email to us

Product Detail

Product Tags

Principle

This kit is designed to detect anti-sperm antibodies (ASAb) in human serum or plasma samples. Its polystyrene microwell strips are pre-coated with sperm antigens, forming the solid-phase reaction platform.

First, the serum or plasma specimens to be tested are added to the wells. If specific antibodies (such as AS-IgG-Ab and some AS-IgM-Ab) are present in the specimens, they will bind to the pre-coated sperm antigens on the solid phase. Unbound components are then removed through washing to eliminate interference.

In the second step, HRP (horseradish peroxidase)-conjugated anti-human IgG is added, which specifically binds to the anti-sperm antibodies already attached to the solid phase. After washing to remove unbound HRP conjugates, TMB substrate solution is added. The HRP in the formed immune complex (sperm antigen-anti-sperm antibody-HRP conjugate) catalyzes the TMB to develop a blue color. Adding 50μl of stop solution turns the color to yellow. Finally, a microplate reader measures the absorbance to determine the presence of AS-IgG antibodies in the sample.

Product Features

High sensitivity, specificity and stability

Product Specification

Principle	Enzyme linked immunosorbent assay
Type	Indirect Method
Certificate	NMPA
Specimen	Human serum / plasma
Specification	48T / 96T
Storage temperature	2-8℃
Shelf life	12 months