Hepatitis B Virus Large Protein ELISA Kit

Short Description:

Hepatitis B virus large surface protein (HBV-LP), also known as the large hepatitis B surface antigen or pre-S1/pre-S2 containing protein, is a component of the viral envelope found predominantly on infectious virions (Dane particles) and filamentous subviral particles. It is expressed at high levels during active HBV replication and serves as a reliable serological marker reflecting the intensity of viral replication at the protein level.

HBV-LP typically appears early in acute infection, often concurrently with or shortly after HBsAg, and its presence correlates strongly with high viral load (HBV DNA) and infectivity. In chronic hepatitis B, persistent detection of HBV-LP indicates ongoing active replication, even in cases where HBeAg has seroconverted to anti-HBe due to precore or core promoter mutations (HBeAg-negative chronic hepatitis B). As such, HBV-LP detection provides a valuable supplementary marker to HBeAg for assessing replicative status, particularly in HBeAg-negative patients where HBeAg alone may underestimate viral activity.

Monitoring HBV-LP is clinically useful for early diagnosis of acute infection, evaluating disease progression, predicting treatment response, and assessing viral suppression during antiviral therapy. Its clearance often precedes or accompanies significant reduction in HBV DNA and may indicate effective control of infection. When combined with conventional markers (HBsAg, HBeAg/anti-HBe, HBV DNA) and pre-S1/pre-S2 antigen testing, HBV-LP detection enhances the comprehensive management of hepatitis B patients.

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Principle:

This kit employs the double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) principle for the qualitative detection of hepatitis B virus large surface protein (HBV-LP), also known as large hepatitis B surface antigen, in human serum or plasma samples. The polystyrene microwell strips are pre-coated with monoclonal antibodies specific for the conformational pre-S domain of HBV-LP (capture antibodies). Upon addition of the serum or plasma specimen, any HBV-LP present in the sample binds specifically to the immobilized capture antibodies during incubation. Unbound components are subsequently removed by washing. In the next step, horseradish peroxidase (HRP)-conjugated anti-HBs antibodies (HBsAb-HRP detection antibodies) are added, which bind to different epitopes on the captured HBV-LP, forming a (capture Ab) - HBV-LP - (HRP-detection Ab) sandwich complex. Following a wash step to remove unbound HRP conjugates, a chromogenic substrate (TMB) is added to the wells. In the presence of the immunocomplex, the HRP enzyme catalyzes the substrate reaction, producing a blue color proportional to the amount of captured HBV-LP. The reaction is terminated by adding a stop solution (typically 50 μL per well), which changes the color to yellow. The absorbance (optical density) is then measured using a microplate reader at the appropriate wavelength (e.g., 450 nm, with a reference wavelength of 620-690 nm if required) to determine the presence or absence of HBV large surface protein in the sample.

Product Features:

High sensitivity, specificity and stability

Product Specification:

Principle	Enzyme linked immunosorbent assay
Type	Double-antibody Sandwich
Certificate	NMPA
Specimen	Human serum / plasma
Specification	96T
Storage temperature	2-8℃
Shelf life	12 months