Coxsackievirus A16 IgM ELISA Kit

This kit employs the capture ELISA principle to detect Coxsackievirus A16 (CA16) IgM antibodies (CA16-IgM) in human serum. Microplate wells are pre-coated with mouse anti-human IgM (μ chain) antibodies, which selectively capture IgM antibodies from the sample. After adding the serum specimen, unbound components—including specific IgG antibodies and other proteins—are removed through washing steps. In the next step, a CA16 antigen-anti-CA16 antibody-enzyme conjugate is introduced. The captured CA16-IgM antibodies specifically bind to the CA16 antigens within the conjugate, forming an immunocomplex: anti-μ chain antibody – CA16-IgM – CA16 antigen-anti-CA16 antibody-HRP conjugate. Unbound conjugates are washed away, and TMB (3,3',5,5'-tetramethylbenzidine) substrate is added to initiate a color reaction catalyzed by horseradish peroxidase (HRP). Adding stop solution turns the blue product yellow, and the absorbance (A value) is measured by a microplate reader. The result, compared to a validated cut-off, determines the presence of CA16-IgM, enabling specific detection of acute CA16 infection. This method minimizes cross-reactivity and ensures reliable clinical diagnosis.