Echovirus IgM ELISA Kit

Short Description:

Echovirus, a human enterovirus, causes encephalitis, aseptic meningitis, myocarditis, pericarditis, epidemic myalgia, and exanthematous diseases in children. After infection, IgM, IgA, and IgG antibodies can be detected. IgM positivity indicates acute infection, while IgG positivity signals past exposure. A 4-fold increase in IgG titer between two blood samples collected 8–10 days apart confirms acute infection. High serum IgG prevalence correlates with repeated infections of different echovirus subtypes in early childhood, making IgG useful for epidemiological studies. Approximately 70% of acute infection cases show elevated IgA titers. Alongside direct viral isolation, IgA antibody testing complements specific IgG detection to enhance diagnostic accuracy for echovirus infections.

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Product Detail

Product Tags

Principle

This kit uses capture ELISA to detect Echovirus IgM (ECHO-IgM) in human serum. Microplate wells are pre-coated with mouse anti-human IgM (μ chain) antibodies. After adding the serum sample, IgM antibodies are captured, and unbound components (including IgG) are washed away. Next, a mixture of Echovirus antigens and anti-Echovirus antibody-enzyme conjugates is added, where captured ECHO-IgM specifically binds to Echovirus antigens. Unbound conjugates are removed by washing, followed by adding TMB substrate for color development. The reaction is stopped by adding a stop solution, turning the color from blue to yellow. The absorbance (A value) is measured by a microplate reader to determine the presence of ECHO-IgM, enabling specific detection of acute Echovirus infection.

Product Features

High sensitivity, specificity and stability

Product Specification

Principle	Enzyme linked immunosorbent assay
Type	Capture Method
Certificate	NMPA
Specimen	Human serum / plasma
Specification	48T / 96T
Storage temperature	2-8℃
Shelf life	12 months