Hepatitis D virus IgG (HDV-IgG) ELISA Kit

Short Description:

Hepatitis D virus (HDV) is a defective, single-stranded RNA virus that requires the hepatitis B virus (HBV) for its replication and assembly, as it relies on HBV’s surface antigen (HBsAg) to form its envelope. It cannot replicate independently and must be accompanied or preceded by HBV infection. HDV is transmitted primarily through parenteral routes, such as exposure to infected blood or blood products (e.g., via transfusion or needle sharing among intravenous drug users), but also through sexual contact, close household contact, and vertical transmission from mother to child. HDV infection has a global distribution, with higher endemicity in regions such as Central and West Africa, the Amazon Basin, Central Asia, the Middle East, and parts of Eastern Europe and the Pacific Islands; prevalence has declined in some areas like Southern Italy due to HBV vaccination and improved hygiene.

Co-infection occurs when HDV and HBV are acquired simultaneously, often leading to acute hepatitis that may resolve or progress to chronicity. Superinfection refers to HDV infection in individuals with pre-existing chronic HBV, which frequently results in more severe outcomes. Clinical studies demonstrate that HDV infection exacerbates HBV-related liver disease, accelerating progression to cirrhosis, liver failure, and hepatocellular carcinoma, and it is a major contributor to fulminant hepatic failure in co-infected or superinfected patients.

The body produces anti-HDV IgG antibodies in response to HDV infection, typically appearing after the initial IgM response and persisting for many years or lifelong. Anti-HDV IgG serves as a marker of past resolved infection, ongoing chronic infection, or immunity (though protective immunity against HDV reinfection is not always complete due to its dependence on HBV). Detection of anti-HDV IgG is particularly useful in screening high-risk populations, confirming prior exposure in HBV carriers, and monitoring disease progression or response to antiviral therapy in chronic cases. In certain diagnostic scenarios, a rise in anti-HDV IgG titers can support the identification of recent superinfection.

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Product Detail

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Principle:

This kit employs the indirect enzyme-linked immunosorbent assay (ELISA) principle to detect hepatitis D virus IgG antibodies (anti-HDV IgG) in human serum or plasma samples. The polystyrene microwell strips are pre-coated with hepatitis D virus antigens. Upon addition of the serum or plasma specimen, any anti-HDV IgG antibodies present in the sample bind specifically to the immobilized antigens, while unbound components are removed by washing. In the subsequent step, horseradish peroxidase (HRP)-conjugated mouse anti-human IgG antibodies are added, which bind to the captured human IgG. Following another wash to remove unbound HRP conjugates, a chromogenic substrate (TMB) is added to the wells. In the presence of the antigen - anti-HDV IgG - HRP-anti-human IgG immunocomplex, the HRP enzyme catalyzes the substrate reaction, producing a blue color that is proportional to the concentration of anti-HDV IgG. The reaction is halted by adding a stop solution (typically 50 μL per well), which shifts the color to yellow. The absorbance (optical density) is then measured using a microplate reader at the appropriate wavelength (e.g., 450 nm) to determine the presence and level of anti-HDV IgG antibodies in the sample.

Product Features:

High sensitivity, specificity and stability

Product Specification:

Principle	Enzyme linked immunosorbent assay
Type	Indirect Method
Certificate	NMPA
Specimen	Human serum / plasma
Specification	48T / 96T
Storage temperature	2-8℃
Shelf life	12 months