Mumps Virus IgM ELISA Kit

This kit is designed for the detection of Mumps virus IgM antibody (MuV-IgM) in human serum or plasma specimens. The assay utilizes polystyrene microwell strips pre-coated with antibodies specific to human immunoglobulin M (anti-μ chain antibodies). The testing process involves a two-step reaction. First, the serum or plasma sample is added to the wells, allowing the anti-μ chain antibodies to capture IgM antibodies present in the specimen. Unbound components, including specific IgG antibodies, are then removed through a washing step. In the second step, horseradish peroxidase (HRP)-conjugated Mumps virus antigens are introduced. These conjugates specifically bind to the captured MuV-IgM antibodies, forming an immunocomplex structure: (anti-μ) - (MuV-IgM) - (MuV-Ag-HRP). After washing away any unbound HRP-conjugates, chromogen solutions containing TMB substrate are added to the wells. The HRP enzyme in the immunocomplex catalyzes the TMB substrate, leading to a color development reaction that initially produces a blue substance. The addition of 50μl Stop Solution converts the blue color to yellow. Finally, the absorbance of the solution in each well is measured using a microplate reader, which helps determine the presence of MuV-IgM antibodies in the sample. This method enables the specific and sensitive detection of recent Mumps virus infection through an enzyme-linked immunosorbent assay (ELISA) format.