TB-IGRA Diagnostic Test

Short Description:

TB-IGRA diagnostic test, also referred to as Interferon Gamma Release Assay, is an ELISA for quantitative detection of Interferon Gamma (IFN-γ) that responds to in vitro stimulation by Mycobacterium Tuberculosis antigens in human blood samples. TB-IGRA measures a person’s immune reactivity to Mycobacterium Tuberculosis. The test is intended for use as an aid in the diagnosis of TB infection including both latent Tuberculosis infection and Tuberculosis disease.

The immune response caused by Mycobacterium tuberculosis is predominantly a cellular response. After infection with Mycobacterium tuberculosis, the body produces specific memory T cells that circulate in the peripheral blood. The measurement of IFN-γ against Mycobacterium tuberculosis has been shown to be an effective technique for detecting TB infection (both latent and active), called IFN-γ in vitro release assay (IGRA). The major difference from the tuberculin test (TST) is that IGRA selects specific antigens that are present only in Mycobacterium tuberculosis but are absent in BCG and most non-tuberculous mycobacteria.

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Product Detail

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Principle

The kit adopts interferon-γ release assay for Mycobacterium tuberculosis (TB-IGRA) to measure the intensity of the cellular immune response mediated by Mycobacterium tuberculosis specific antigen.
The Enzyme-linked immunosorbent assay and double antibody sandwich principle.
• The microplates are pre-coated with anti IFN-γ antibodies.
• The specimens to be tested are added into the antibody coated microplate wells, then horseradish peroxidase (HRP)-conjugated anti IFN-γ antibodies are added into the respective wells.
• IFN-γ, if present, will form a sandwich complex with anti IFN-γ antibodies and HRP-conjugated anti IFN-γ antibodies.
• Color will be developed after adding the substrate solutions, and will change after adding the stop solutions. The absorbance (OD) is measured with an ELISA reader.
• The IFN-γ concentration in the sample is correlated to the determined OD.

Product Features

Effective diagnostic ELISA for latent and active TB infection

No interference from BCG vaccine

Product Specification

Principle	Enzyme linked immunosorbent assay
Type	Sandwich method
Certificate	CE，NMPA
Specimen	Whole blood
Specification	48T (detect 11 samples); 96T (detect 27 samples)
Storage temperature	2-8℃
Shelf life	12 months